Structural heterogeneity of phospholipase D in 10 dicots

Jim Dyer, Suqin Zheng, Xuemin Wang

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

The occurrence of multiple forms of phospholipase D (EC 3.1.4.4) was investigated in different tissues of castor bran (Ricinus communis) and in other plant species. Phospholipase D variants were resolved by nondenaturing and isoelectric focusing polyacrylamide gel electrophoresis and detected by immunoblotting using anti-phospholipase D antibodies and by enzyme activity assay. Three phospholipase D variants were produced differentially in the roots, endosperm, cotyledons, and hypocotyl of 5-day postgermination seedlings of castor bean. Furthermore, different patterns of phospholipase D variants were found in the different regions of hypocotyl (elongated and hook). Multiple phospholipase D forms were found in florets of cauliflower and broccoli, leaves of cabbage, celery, tomato, and potato, and alfalfa sprouts, suggesting that structural heterogeneity of phospholipase D occurs widely in plants.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number1
DOIs
StatePublished - 5 Apr 1996

Fingerprint

Phospholipase D
Brassica
Hypocotyl
4 alpha-glucanotransferase
Castor Bean
Apium graveolens
Ricinus
Endosperm
Medicago sativa
Cotyledon
Hooks
Enzyme Assays
Enzyme activity
Isoelectric Focusing
Lycopersicon esculentum
Solanum tuberosum
Electrophoresis
Seedlings
Immunoblotting
Polyacrylamide Gel Electrophoresis

Cite this

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title = "Structural heterogeneity of phospholipase D in 10 dicots",
abstract = "The occurrence of multiple forms of phospholipase D (EC 3.1.4.4) was investigated in different tissues of castor bran (Ricinus communis) and in other plant species. Phospholipase D variants were resolved by nondenaturing and isoelectric focusing polyacrylamide gel electrophoresis and detected by immunoblotting using anti-phospholipase D antibodies and by enzyme activity assay. Three phospholipase D variants were produced differentially in the roots, endosperm, cotyledons, and hypocotyl of 5-day postgermination seedlings of castor bean. Furthermore, different patterns of phospholipase D variants were found in the different regions of hypocotyl (elongated and hook). Multiple phospholipase D forms were found in florets of cauliflower and broccoli, leaves of cabbage, celery, tomato, and potato, and alfalfa sprouts, suggesting that structural heterogeneity of phospholipase D occurs widely in plants.",
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Structural heterogeneity of phospholipase D in 10 dicots. / Dyer, Jim; Zheng, Suqin; Wang, Xuemin.

In: Biochemical and Biophysical Research Communications, Vol. 221, No. 1, 05.04.1996, p. 31-36.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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