Structural heterogeneity of phospholipase D in 10 dicots

James H. Dyer, Suqin Zheng, Xuemin Wang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The occurrence of multiple forms of phospholipase D (EC 3.1.4.4) was investigated in different tissues of castor bran (Ricinus communis) and in other plant species. Phospholipase D variants were resolved by nondenaturing and isoelectric focusing polyacrylamide gel electrophoresis and detected by immunoblotting using anti-phospholipase D antibodies and by enzyme activity assay. Three phospholipase D variants were produced differentially in the roots, endosperm, cotyledons, and hypocotyl of 5-day postgermination seedlings of castor bean. Furthermore, different patterns of phospholipase D variants were found in the different regions of hypocotyl (elongated and hook). Multiple phospholipase D forms were found in florets of cauliflower and broccoli, leaves of cabbage, celery, tomato, and potato, and alfalfa sprouts, suggesting that structural heterogeneity of phospholipase D occurs widely in plants.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number1
DOIs
StatePublished - 5 Apr 1996

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