Subcellular localization of chicken kidney aryl acylamidase activity.

J. J. Gaynor, C. C. Still

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The intracellular localization of aryl acylamidase (aryl-acylamide amidohydrolase, EC 3.5.1.13) in chicken kidney was investigated. By separation on density gradients of the silica sol Ludox AM, the enzyme was localized in the mitochondrial fraction. This mitochondrial fraction was shown to be substantially free of lysosomal contamination. Subfractionation of the purified mitochondria indicates that the enzyme is located on the outer membrane, can be solubilized, and may be a suitable marker enzyme for kidney mitochondria.

Original languageEnglish
Pages (from-to)507-513
Number of pages7
JournalThe Biochemical journal
Volume186
Issue number2
DOIs
StatePublished - 1 Jan 1980

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