Substrate binding modulates the reduction potential of DNA photolyase

Yvonne M. Gindt, Johannes P.M. Schelvis, Katie L. Thoren, Tina H. Huang

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


The reduction potential of the (FADH-/FADH) couple in DNA photolyase was measured, and the value was found to be significantly higher than the values estimated in the literature. In the absence of substrate, the enzyme has a reduction potential of 16 ± 6 mV vs NHE. In the presence of excess substrate the reduction potential increases to 81 ± 8 mV vs NHE. The increase in reduction potential has physiological relevance since it gives the catalytic state greater resistance to oxidation. This is the first measurement of a reduction potential for this class of DNA-repair enzymes and the larger family of blue-light photoreceptors.

Original languageEnglish
Pages (from-to)10472-10473
Number of pages2
JournalJournal of the American Chemical Society
Issue number30
StatePublished - 3 Aug 2005


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