Substrate binding modulates the reduction potential of DNA photolyase

Yvonne Gindt, Johannes Schelvis, Katie L. Thoren, Tina H. Huang

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)

Abstract

The reduction potential of the (FADH-/FADH) couple in DNA photolyase was measured, and the value was found to be significantly higher than the values estimated in the literature. In the absence of substrate, the enzyme has a reduction potential of 16 ± 6 mV vs NHE. In the presence of excess substrate the reduction potential increases to 81 ± 8 mV vs NHE. The increase in reduction potential has physiological relevance since it gives the catalytic state greater resistance to oxidation. This is the first measurement of a reduction potential for this class of DNA-repair enzymes and the larger family of blue-light photoreceptors.

Original languageEnglish
Pages (from-to)10472-10473
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number30
DOIs
StatePublished - 3 Aug 2005

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Deoxyribodipyrimidine Photo-Lyase
DNA Repair Enzymes
DNA
Light
Substrates
Enzymes
Oxidation

Cite this

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Substrate binding modulates the reduction potential of DNA photolyase. / Gindt, Yvonne; Schelvis, Johannes; Thoren, Katie L.; Huang, Tina H.

In: Journal of the American Chemical Society, Vol. 127, No. 30, 03.08.2005, p. 10472-10473.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Substrate binding modulates the reduction potential of DNA photolyase

AU - Gindt, Yvonne

AU - Schelvis, Johannes

AU - Thoren, Katie L.

AU - Huang, Tina H.

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