The auxin conjugate hydrolase family of Medicago truncatula and their expression during the interaction with two symbionts

James Campanella, Stephanie M. Smith, Dora Leibu, Shiri Wexler, Jutta Ludwig-Müller

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We have characterized the regulation of auxin conjugate hydrolysis in Medicago truncatula during development and interaction with two symbionts. In Medicago truncatula five putative auxin amidohydrolase genes (MtIAR31, MtIAR32, MtIAR33, MtIAR34, MtIAR36) were identified, homologous to the AtIAR3 gene from Arabidopsis thaliana. The MtIAR32 transcript is the most abundant transcript in whole seedlings after germination, whereas MtIAR33 is the least abundant. MtIAR32 is also the most abundant transcript in adult roots, stems, and basal leaves. MtIAR31 is most highly expressed in flowers and MtIAR36 in terminal leaves. Sinorhizobium meliloti-infected seedlings primarily upregulated MtIAR33 and -34 transcripts. Glomus intraradices-infected seedlings upregulated MtIAR33 and -34. MtIAR31, -32, -33, and -34 have hydrolytic activity against IAA-aspartate and IBA-alanine. MtIAR33, -34, and -36 hydrolyze the ester bonds of IAA-glucose. MtIAR36 solely possesses activity against IAA-glycine, -alanine, and -isoleucine. IBA was increased in Glomus intraradices-inoculated roots of Medicago compared to controls, whereas IAA was not. Our results suggest an intricate control system that regulates free and conjugated auxins in Medicago.

Original languageEnglish
Pages (from-to)26-38
Number of pages13
JournalJournal of Plant Growth Regulation
Volume27
Issue number1
DOIs
StatePublished - 1 Mar 2008

Fingerprint

Medicago truncatula
hydrolases
symbionts
indole acetic acid
auxins
Medicago
Glomus intraradices
indole butyric acid
alanine
seedlings
amide hydrolases
Ensifer meliloti
aspartic acid
isoleucine
glycine (amino acid)
leaves
genes
Arabidopsis thaliana
hydrolysis
esters

Keywords

  • Arbuscular mycorrhiza
  • Auxin
  • Auxin amidohydrolases
  • Medicago truncatula
  • Nodulation
  • Symbiosis

Cite this

Campanella, James ; Smith, Stephanie M. ; Leibu, Dora ; Wexler, Shiri ; Ludwig-Müller, Jutta. / The auxin conjugate hydrolase family of Medicago truncatula and their expression during the interaction with two symbionts. In: Journal of Plant Growth Regulation. 2008 ; Vol. 27, No. 1. pp. 26-38.
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The auxin conjugate hydrolase family of Medicago truncatula and their expression during the interaction with two symbionts. / Campanella, James; Smith, Stephanie M.; Leibu, Dora; Wexler, Shiri; Ludwig-Müller, Jutta.

In: Journal of Plant Growth Regulation, Vol. 27, No. 1, 01.03.2008, p. 26-38.

Research output: Contribution to journalArticle

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T1 - The auxin conjugate hydrolase family of Medicago truncatula and their expression during the interaction with two symbionts

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AB - We have characterized the regulation of auxin conjugate hydrolysis in Medicago truncatula during development and interaction with two symbionts. In Medicago truncatula five putative auxin amidohydrolase genes (MtIAR31, MtIAR32, MtIAR33, MtIAR34, MtIAR36) were identified, homologous to the AtIAR3 gene from Arabidopsis thaliana. The MtIAR32 transcript is the most abundant transcript in whole seedlings after germination, whereas MtIAR33 is the least abundant. MtIAR32 is also the most abundant transcript in adult roots, stems, and basal leaves. MtIAR31 is most highly expressed in flowers and MtIAR36 in terminal leaves. Sinorhizobium meliloti-infected seedlings primarily upregulated MtIAR33 and -34 transcripts. Glomus intraradices-infected seedlings upregulated MtIAR33 and -34. MtIAR31, -32, -33, and -34 have hydrolytic activity against IAA-aspartate and IBA-alanine. MtIAR33, -34, and -36 hydrolyze the ester bonds of IAA-glucose. MtIAR36 solely possesses activity against IAA-glycine, -alanine, and -isoleucine. IBA was increased in Glomus intraradices-inoculated roots of Medicago compared to controls, whereas IAA was not. Our results suggest an intricate control system that regulates free and conjugated auxins in Medicago.

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