TY - JOUR
T1 - The Coxsackievirus and Adenovirus Receptor Has a Short Half-Life in Epithelial Cells
AU - Narayan, Poornima Kotha Lakshmi
AU - Readler, James M.
AU - Alghamri, Mahmoud S.
AU - Brockman, Trisha L.
AU - Yan, Ran
AU - Sharma, Priyanka
AU - Snitsarev, Vladislav
AU - Excoffon, Katherine J.D.A.
AU - Kolawole, Abimbola O.
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2
Y1 - 2022/2
N2 - The coxsackievirus and adenovirus receptor (CAR) is an essential cellular protein that is involved in cell adhesion, cell signaling, and viral infection. The 8-exon encoded isoform (CAREx8) resides at the apical surface of polarized epithelia, where it is accessible as a receptor for adenovirus entering the airway lumen. Given its pivotal role in viral infection, it is a target for antiviral strategies. To understand the regulation of CAREx8 and determine the feasibility of receptor downregulation, the half-life of total and apical localized CAREx8 was determined and correlated with adenovirus transduction. Total and apical CAREx8 has a relatively short half-life of approximately 2 h. The half-life of apical CAREx8 correlates well with adenovirus transduction. These results suggest that antiviral strategies that aim to degrade the primary receptor for apical adenovirus infection will be effective within a relatively short time frame after application.
AB - The coxsackievirus and adenovirus receptor (CAR) is an essential cellular protein that is involved in cell adhesion, cell signaling, and viral infection. The 8-exon encoded isoform (CAREx8) resides at the apical surface of polarized epithelia, where it is accessible as a receptor for adenovirus entering the airway lumen. Given its pivotal role in viral infection, it is a target for antiviral strategies. To understand the regulation of CAREx8 and determine the feasibility of receptor downregulation, the half-life of total and apical localized CAREx8 was determined and correlated with adenovirus transduction. Total and apical CAREx8 has a relatively short half-life of approximately 2 h. The half-life of apical CAREx8 correlates well with adenovirus transduction. These results suggest that antiviral strategies that aim to degrade the primary receptor for apical adenovirus infection will be effective within a relatively short time frame after application.
KW - Coxsackievirus and adenovirus receptor
KW - Half-life
KW - Human adenovirus
KW - Polarized epithelia
UR - http://www.scopus.com/inward/record.url?scp=85124036871&partnerID=8YFLogxK
U2 - 10.3390/pathogens11020173
DO - 10.3390/pathogens11020173
M3 - Article
AN - SCOPUS:85124036871
SN - 2076-0817
VL - 11
JO - Pathogens
JF - Pathogens
IS - 2
M1 - 173
ER -
