The droplet technique: measurement of calcium extrusion from single isolated mammalian cells

A. V. Tepikin, J. Llopis, Vladislav Snitsarev, D. V. Gallacher, O. H. Petersen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.

Original languageEnglish
Pages (from-to)664-670
Number of pages7
JournalPflügers Archiv European Journal of Physiology
Volume428
Issue number5-6
DOIs
StatePublished - 1 Oct 1994

Fingerprint

Extrusion
Cells
Calcium
Acinar Cells
Calibration
Optical properties

Keywords

  • Acinar cells
  • Ca extrusion
  • Ca measurements
  • Droplet technique

Cite this

Tepikin, A. V. ; Llopis, J. ; Snitsarev, Vladislav ; Gallacher, D. V. ; Petersen, O. H. / The droplet technique : measurement of calcium extrusion from single isolated mammalian cells. In: Pflügers Archiv European Journal of Physiology. 1994 ; Vol. 428, No. 5-6. pp. 664-670.
@article{7f0340757b01485496be6f959546cbf9,
title = "The droplet technique: measurement of calcium extrusion from single isolated mammalian cells",
abstract = "This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.",
keywords = "Acinar cells, Ca extrusion, Ca measurements, Droplet technique",
author = "Tepikin, {A. V.} and J. Llopis and Vladislav Snitsarev and Gallacher, {D. V.} and Petersen, {O. H.}",
year = "1994",
month = "10",
day = "1",
doi = "10.1007/BF00374591",
language = "English",
volume = "428",
pages = "664--670",
journal = "Pfl{\"u}gers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "5-6",

}

The droplet technique : measurement of calcium extrusion from single isolated mammalian cells. / Tepikin, A. V.; Llopis, J.; Snitsarev, Vladislav; Gallacher, D. V.; Petersen, O. H.

In: Pflügers Archiv European Journal of Physiology, Vol. 428, No. 5-6, 01.10.1994, p. 664-670.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The droplet technique

T2 - measurement of calcium extrusion from single isolated mammalian cells

AU - Tepikin, A. V.

AU - Llopis, J.

AU - Snitsarev, Vladislav

AU - Gallacher, D. V.

AU - Petersen, O. H.

PY - 1994/10/1

Y1 - 1994/10/1

N2 - This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.

AB - This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.

KW - Acinar cells

KW - Ca extrusion

KW - Ca measurements

KW - Droplet technique

UR - http://www.scopus.com/inward/record.url?scp=0027939035&partnerID=8YFLogxK

U2 - 10.1007/BF00374591

DO - 10.1007/BF00374591

M3 - Article

C2 - 7838690

AN - SCOPUS:0027939035

VL - 428

SP - 664

EP - 670

JO - Pflügers Archiv European Journal of Physiology

JF - Pflügers Archiv European Journal of Physiology

SN - 0031-6768

IS - 5-6

ER -