The droplet technique: measurement of calcium extrusion from single isolated mammalian cells

A. V. Tepikin, J. Llopis, V. A. Snitsarev, D. V. Gallacher, O. H. Petersen

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

This paper contains a description of the modified droplet technique that is designed to monitor Ca2+ extrusion from single isolated pancreatic acinar cells. A cell loaded with calcium indicator is maintained in a small droplet of solution containing another calcium indicator. Differences in the optical properties of the intracellular and extracellular indicators allows one to monitor simultaneously intracellular and extracellular calcium concentrations. The paper contains a description of the calibration procedure that is used to calculate intracellular and extracellular calcium concentrations. The advantages and disadvantages of different pairs of extracellular and intracellular indicators are discussed.

Original languageEnglish
Pages (from-to)664-670
Number of pages7
JournalPflügers Archiv European Journal of Physiology
Volume428
Issue number5-6
DOIs
StatePublished - 1 Oct 1994

Keywords

  • Acinar cells
  • Ca extrusion
  • Ca measurements
  • Droplet technique

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