The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan, Tibor Kiss, Vladislav Snitsarev, Maxim V. Storozhuk, Oleg N. Osipenko

Research output: Contribution to journalArticle

Abstract

The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

Original languageEnglish
Pages (from-to)551-559
Number of pages9
JournalCellular Signalling
Volume6
Issue number5
DOIs
StatePublished - 1 Jan 1994

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Acetylcholine
Serotonin
Calcium
Neurons
Inhibitory Concentration 50
Helix (Snails)
GTP-Binding Proteins
Membranes

Keywords

  • G-proteins
  • Serotonin, acetylcholine
  • cAMP
  • calcium fluxes
  • intracellular calcium
  • ionic currents
  • molluscan identified neuron

Cite this

Belan, Pavel V. ; Kiss, Tibor ; Snitsarev, Vladislav ; Storozhuk, Maxim V. ; Osipenko, Oleg N. / The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons. In: Cellular Signalling. 1994 ; Vol. 6, No. 5. pp. 551-559.
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The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons. / Belan, Pavel V.; Kiss, Tibor; Snitsarev, Vladislav; Storozhuk, Maxim V.; Osipenko, Oleg N.

In: Cellular Signalling, Vol. 6, No. 5, 01.01.1994, p. 551-559.

Research output: Contribution to journalArticle

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T1 - The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

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AU - Kiss, Tibor

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AU - Osipenko, Oleg N.

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