Abstract
The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).
| Original language | English |
|---|---|
| Pages (from-to) | 551-559 |
| Number of pages | 9 |
| Journal | Cellular Signalling |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jul 1994 |
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Keywords
- G-proteins
- Serotonin, acetylcholine
- cAMP
- calcium fluxes
- intracellular calcium
- ionic currents
- molluscan identified neuron
Cite this
}
The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons. / Belan, Pavel V.; Kiss, Tibor; Snitsarev, Vladislav; Storozhuk, Maxim V.; Osipenko, Oleg N.
In: Cellular Signalling, Vol. 6, No. 5, 07.1994, p. 551-559.Research output: Contribution to journal › Article
TY - JOUR
T1 - The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons
AU - Belan, Pavel V.
AU - Kiss, Tibor
AU - Snitsarev, Vladislav
AU - Storozhuk, Maxim V.
AU - Osipenko, Oleg N.
PY - 1994/7
Y1 - 1994/7
N2 - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).
AB - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).
KW - G-proteins
KW - Serotonin, acetylcholine
KW - cAMP
KW - calcium fluxes
KW - intracellular calcium
KW - ionic currents
KW - molluscan identified neuron
UR - http://www.scopus.com/inward/record.url?scp=0028070295&partnerID=8YFLogxK
U2 - 10.1016/0898-6568(94)90009-4
DO - 10.1016/0898-6568(94)90009-4
M3 - Article
C2 - 7818991
AN - SCOPUS:0028070295
VL - 6
SP - 551
EP - 559
JO - Cellular Signalling
JF - Cellular Signalling
SN - 0898-6568
IS - 5
ER -