The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan; Tibor Kiss; Vladislav Snitsarev; Maxim V. Storozhuk; Oleg N. Osipenko

doi:10.1016/0898-6568(94)90009-4

The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan, Tibor Kiss, Vladislav Snitsarev, Maxim V. Storozhuk, Oleg N. Osipenko

Biology

Research output: Contribution to journal › Article

Abstract

The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca²⁺]_in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca²⁺]_in changes is the suppression of calcium inward currents (I_Ca). Influence of Ach and 5-HT on I_Ca were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC₅₀) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in I_Ca (IC₅₀ = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

Original language	English
Pages (from-to)	551-559
Number of pages	9
Journal	Cellular Signalling
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/0898-6568(94)90009-4
Publication status	Published - Jul 1994

Fingerprint

Keywords

G-proteins
Serotonin, acetylcholine
cAMP
calcium fluxes
intracellular calcium
ionic currents
molluscan identified neuron

Cite this

Belan, P. V., Kiss, T., Snitsarev, V., Storozhuk, M. V., & Osipenko, O. N. (1994). The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons. Cellular Signalling, 6(5), 551-559. https://doi.org/10.1016/0898-6568(94)90009-4

@article{631dd1269705480788dcc109e9680130,

title = "The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons",

abstract = "The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).",

keywords = "G-proteins, Serotonin, acetylcholine, cAMP, calcium fluxes, intracellular calcium, ionic currents, molluscan identified neuron",

author = "Belan, {Pavel V.} and Tibor Kiss and Vladislav Snitsarev and Storozhuk, {Maxim V.} and Osipenko, {Oleg N.}",

year = "1994",

month = "7",

doi = "10.1016/0898-6568(94)90009-4",

language = "English",

volume = "6",

pages = "551--559",

journal = "Cellular Signalling",

issn = "0898-6568",

publisher = "Elsevier Inc.",

number = "5",

}

TY - JOUR

T1 - The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

AU - Belan, Pavel V.

AU - Kiss, Tibor

AU - Snitsarev, Vladislav

AU - Storozhuk, Maxim V.

AU - Osipenko, Oleg N.

PY - 1994/7

Y1 - 1994/7

N2 - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

AB - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

KW - G-proteins

KW - Serotonin, acetylcholine

KW - cAMP

KW - calcium fluxes

KW - intracellular calcium

KW - ionic currents

KW - molluscan identified neuron

UR - http://www.scopus.com/inward/record.url?scp=0028070295&partnerID=8YFLogxK

U2 - 10.1016/0898-6568(94)90009-4

DO - 10.1016/0898-6568(94)90009-4

M3 - Article

C2 - 7818991

AN - SCOPUS:0028070295

VL - 6

SP - 551

EP - 559

JO - Cellular Signalling

JF - Cellular Signalling

SN - 0898-6568

IS - 5

ER -

Access to Document

10.1016/0898-6568(94)90009-4

Link to publication in Scopus