The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan; Tibor Kiss; Vladislav Snitsarev; Maxim V. Storozhuk; Oleg N. Osipenko

doi:10.1016/0898-6568(94)90009-4

The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan, Tibor Kiss, Vladislav Snitsarev, Maxim V. Storozhuk, Oleg N. Osipenko

Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca²⁺]_in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca²⁺]_in changes is the suppression of calcium inward currents (I_Ca). Influence of Ach and 5-HT on I_Ca were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC₅₀) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in I_Ca (IC₅₀ = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

Original language	English
Pages (from-to)	551-559
Number of pages	9
Journal	Cellular Signalling
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/0898-6568(94)90009-4
State	Published - Jul 1994

Keywords

G-proteins
Serotonin, acetylcholine
cAMP
calcium fluxes
intracellular calcium
ionic currents
molluscan identified neuron

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title = "The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons",

abstract = "The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).",

keywords = "G-proteins, Serotonin, acetylcholine, cAMP, calcium fluxes, intracellular calcium, ionic currents, molluscan identified neuron",

author = "Belan, {Pavel V.} and Tibor Kiss and Vladislav Snitsarev and Storozhuk, {Maxim V.} and Osipenko, {Oleg N.}",

year = "1994",

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T1 - The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

AU - Belan, Pavel V.

AU - Kiss, Tibor

AU - Snitsarev, Vladislav

AU - Storozhuk, Maxim V.

AU - Osipenko, Oleg N.

PY - 1994/7

Y1 - 1994/7

N2 - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

AB - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

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KW - Serotonin, acetylcholine

KW - cAMP

KW - calcium fluxes

KW - intracellular calcium

KW - ionic currents

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The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

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