The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan; Tibor Kiss; Vladislav Snitsarev; Maxim V. Storozhuk; Oleg N. Osipenko

doi:10.1016/0898-6568(94)90009-4

The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Pavel V. Belan
, Tibor Kiss
, Vladislav Snitsarev
, Maxim V. Storozhuk
, Oleg N. Osipenko

Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca²⁺]_in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca²⁺]_in changes is the suppression of calcium inward currents (I_Ca). Influence of Ach and 5-HT on I_Ca were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC₅₀) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in I_Ca (IC₅₀ = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

Original language	English
Pages (from-to)	551-559
Number of pages	9
Journal	Cellular Signalling
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/0898-6568(94)90009-4
State	Published - Jul 1994

Keywords

G-proteins
Serotonin, acetylcholine
cAMP
calcium fluxes
intracellular calcium
ionic currents
molluscan identified neuron

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10.1016/0898-6568(94)90009-4

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title = "The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons",

abstract = "The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).",

keywords = "G-proteins, Serotonin, acetylcholine, cAMP, calcium fluxes, intracellular calcium, ionic currents, molluscan identified neuron",

author = "Belan, \{Pavel V.\} and Tibor Kiss and Vladislav Snitsarev and Storozhuk, \{Maxim V.\} and Osipenko, \{Oleg N.\}",

year = "1994",

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T1 - The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

AU - Belan, Pavel V.

AU - Kiss, Tibor

AU - Snitsarev, Vladislav

AU - Storozhuk, Maxim V.

AU - Osipenko, Oleg N.

PY - 1994/7

Y1 - 1994/7

N2 - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

AB - The results presented demonstrate that in D neurons of the snail Helix pomatia L., acetylcholine (ACh) (10 ÷ 100 μM) and sertonin (5-HT) (0.1 ÷ 1000 μM) applications reduce both the basal intracellular concentration level ([Ca2+]in) and the amplitudes of calcium transients induced by membrane depolatrization. It is likely that the mechanism of [Ca2+]in changes is the suppression of calcium inward currents (ICa). Influence of Ach and 5-HT on ICa were studied. Both effects were dose-dependent (ACh-0.01 ÷ μM and 5-HT-0.1 ÷ 1000 μM). The half-maximal effects (IC50) were evoked by Ah concentration of 0.15 μM and 5-HT-15 μM. Furthermore we have also shown that in some cells 5-HT could evoke a transient increase in ICa (IC50 = 2 μM. The effects of Ach abd 5-HT were nonadditive-the subsequent application of ACh after 5-HT, and vice versa, produced no inhibitory effects. This may indicate that both substances act through a common inermediate (possibly, G-protein).

KW - G-proteins

KW - Serotonin, acetylcholine

KW - cAMP

KW - calcium fluxes

KW - intracellular calcium

KW - ionic currents

KW - molluscan identified neuron

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U2 - 10.1016/0898-6568(94)90009-4

DO - 10.1016/0898-6568(94)90009-4

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AN - SCOPUS:0028070295

SN - 0898-6568

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EP - 559

JO - Cellular Signalling

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ER -

The effect of acetylcholine and serotonin on calcium transient and calcium currents in identified Helix pomatia L. neurons

Abstract

Keywords

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