The immunophilin ligand rapamycin: A probe for the analysis of the relationship of apoptosis to the cell cycle

Scott Wadsworth, John Siekierka

Research output: Contribution to journalArticle

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Abstract

A common consequence of growth factor deprivation in actively proliferating cells is arrest in the G1 phase of the cell cycle and subsequent apoptosis (programmed cell death). We have utilized the potent cell cycle inhibitor rapamycin (Rapa) to define move precisely the point within G1 where apoptosis is initiated during IL-2 deprivation in murine CTLL cells. Our results indicate that apoptosis is not initiated at a single point within G1, but rather can be initiated in at least two distinct points of the cell cycle, early and late G1. In nonproliferating CTLL cells arrested with Rapa in late G1, IL-2 prevents the initiation of an apoptotic response. This suggests that an IL-2-mediated signal is necessary to prevent apoptosis when proliferation is blocked, whether the block to proliferation arrests cells in early or late G1.

Original languageEnglish
Pages (from-to)160-164
Number of pages5
JournalMethods: A Companion to Methods in Enzymology
Volume9
Issue number2
DOIs
StatePublished - 1 Jan 1996

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Immunophilins
Sirolimus
Cell Cycle
Cells
Apoptosis
Ligands
Interleukin-2
Cell proliferation
G1 Phase
Cell death
Intercellular Signaling Peptides and Proteins
Cell Death
Cell Proliferation

Cite this

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The immunophilin ligand rapamycin : A probe for the analysis of the relationship of apoptosis to the cell cycle. / Wadsworth, Scott; Siekierka, John.

In: Methods: A Companion to Methods in Enzymology, Vol. 9, No. 2, 01.01.1996, p. 160-164.

Research output: Contribution to journalArticle

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