The influence of NMDA and GABAA receptors and glutamic acid decarboxylase (GAD) activity on attention

Alan Pehrson, Corina O. Bondi, Nelson K.B. Totah, Bita Moghaddam

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

Rationale: Attention dysfunction is the hallmark of cognitive deficits associated with major psychiatric illnesses including schizophrenia. Cognitive deficits of schizophrenia have been attributed to reduced function of the N-methyl-d-aspartate (NMDA) receptor or reduced expression of the gamma-aminobutyric acid (GABA)-synthesizing enzyme glutamic acid decarboxylase-67, which presumably leads to attenuated neurotransmission at GABAA receptors. Objective: The present study used a rodent model to compare the inhibition of NMDA and GABAA receptors, and GAD activity on attention. We tested the impact of inhibiting these proteins brain wide or in the anterior cingulate cortex (ACC), a prefrontal cortex region critical for attentional processing. Methods: Rats were trained on the three choice serial reaction time task (3-CSRT), an attention test. The impact of systemic or intra-ACC injection of drugs on performance was measured in well-trained rats. Results: Reducing GABAA receptor function within the ACC with the direct antagonist SR95531 (1 or 3 ng/side) or brain wide using systemic injection of the benzodiazepine inverse agonist FG7142 (5 mg/kg) impaired accuracy and increased omissions. Systemic or intra-ACC inhibition of NMDA receptors using MK-801 (at 3 mg/kg or 3 μg, respectively) also impaired performance. Inhibition of GAD with 3-mercaptopropionic acid, even at high doses, had no effect on 3-CSRT accuracy or omissions when administered systemically or within the ACC. Conclusions: These data demonstrate that, while tonic stimulation of NMDA and GABAA receptors within the ACC are critical for attentional performance, reduction in GAD activity may have little functional significance and is not indicative of reduced GABA neurotransmission.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalPsychopharmacology
Volume225
Issue number1
DOIs
StatePublished - 1 Jan 2013

Fingerprint

Glutamate Decarboxylase
Gyrus Cinguli
GABA-A Receptors
Synaptic Transmission
gamma-Aminobutyric Acid
Schizophrenia
3-Mercaptopropionic Acid
Injections
Dizocilpine Maleate
Brain
Prefrontal Cortex
Benzodiazepines
Reaction Time
Psychiatry
aspartic acid receptor
Rodentia
Enzymes
Pharmaceutical Preparations
Inhibition (Psychology)
Proteins

Keywords

  • Attention
  • GABA
  • Glutamate
  • Glutamic acid decarboxylase
  • Schizophrenia

Cite this

Pehrson, Alan ; Bondi, Corina O. ; Totah, Nelson K.B. ; Moghaddam, Bita. / The influence of NMDA and GABAA receptors and glutamic acid decarboxylase (GAD) activity on attention. In: Psychopharmacology. 2013 ; Vol. 225, No. 1. pp. 31-39.
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The influence of NMDA and GABAA receptors and glutamic acid decarboxylase (GAD) activity on attention. / Pehrson, Alan; Bondi, Corina O.; Totah, Nelson K.B.; Moghaddam, Bita.

In: Psychopharmacology, Vol. 225, No. 1, 01.01.2013, p. 31-39.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The influence of NMDA and GABAA receptors and glutamic acid decarboxylase (GAD) activity on attention

AU - Pehrson, Alan

AU - Bondi, Corina O.

AU - Totah, Nelson K.B.

AU - Moghaddam, Bita

PY - 2013/1/1

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N2 - Rationale: Attention dysfunction is the hallmark of cognitive deficits associated with major psychiatric illnesses including schizophrenia. Cognitive deficits of schizophrenia have been attributed to reduced function of the N-methyl-d-aspartate (NMDA) receptor or reduced expression of the gamma-aminobutyric acid (GABA)-synthesizing enzyme glutamic acid decarboxylase-67, which presumably leads to attenuated neurotransmission at GABAA receptors. Objective: The present study used a rodent model to compare the inhibition of NMDA and GABAA receptors, and GAD activity on attention. We tested the impact of inhibiting these proteins brain wide or in the anterior cingulate cortex (ACC), a prefrontal cortex region critical for attentional processing. Methods: Rats were trained on the three choice serial reaction time task (3-CSRT), an attention test. The impact of systemic or intra-ACC injection of drugs on performance was measured in well-trained rats. Results: Reducing GABAA receptor function within the ACC with the direct antagonist SR95531 (1 or 3 ng/side) or brain wide using systemic injection of the benzodiazepine inverse agonist FG7142 (5 mg/kg) impaired accuracy and increased omissions. Systemic or intra-ACC inhibition of NMDA receptors using MK-801 (at 3 mg/kg or 3 μg, respectively) also impaired performance. Inhibition of GAD with 3-mercaptopropionic acid, even at high doses, had no effect on 3-CSRT accuracy or omissions when administered systemically or within the ACC. Conclusions: These data demonstrate that, while tonic stimulation of NMDA and GABAA receptors within the ACC are critical for attentional performance, reduction in GAD activity may have little functional significance and is not indicative of reduced GABA neurotransmission.

AB - Rationale: Attention dysfunction is the hallmark of cognitive deficits associated with major psychiatric illnesses including schizophrenia. Cognitive deficits of schizophrenia have been attributed to reduced function of the N-methyl-d-aspartate (NMDA) receptor or reduced expression of the gamma-aminobutyric acid (GABA)-synthesizing enzyme glutamic acid decarboxylase-67, which presumably leads to attenuated neurotransmission at GABAA receptors. Objective: The present study used a rodent model to compare the inhibition of NMDA and GABAA receptors, and GAD activity on attention. We tested the impact of inhibiting these proteins brain wide or in the anterior cingulate cortex (ACC), a prefrontal cortex region critical for attentional processing. Methods: Rats were trained on the three choice serial reaction time task (3-CSRT), an attention test. The impact of systemic or intra-ACC injection of drugs on performance was measured in well-trained rats. Results: Reducing GABAA receptor function within the ACC with the direct antagonist SR95531 (1 or 3 ng/side) or brain wide using systemic injection of the benzodiazepine inverse agonist FG7142 (5 mg/kg) impaired accuracy and increased omissions. Systemic or intra-ACC inhibition of NMDA receptors using MK-801 (at 3 mg/kg or 3 μg, respectively) also impaired performance. Inhibition of GAD with 3-mercaptopropionic acid, even at high doses, had no effect on 3-CSRT accuracy or omissions when administered systemically or within the ACC. Conclusions: These data demonstrate that, while tonic stimulation of NMDA and GABAA receptors within the ACC are critical for attentional performance, reduction in GAD activity may have little functional significance and is not indicative of reduced GABA neurotransmission.

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KW - Glutamate

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U2 - 10.1007/s00213-012-2792-z

DO - 10.1007/s00213-012-2792-z

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