The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel

Dattananda S. Chelur, Glen G. Ernstrom, Miriam B. Goodman, C. Andrea Yao, Lei Chen, Robert O'Hagan, Martin Chalfie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Mechanosensory transduction in touch receptor neurons is believed to be mediated by DEG/ENaC (degenerin/epithelial Na+ channel) proteins in nematodes and mammals. In the nematode Caenorhabditis elegans, gain-of-function mutations in the degenerin genes mec-4 and mec-10 (denoted mec-4(d) and mec-10(d), respectively) cause degeneration of the touch cells. This phenotype is completely suppressed by mutation in a third gene, mec-6 (refs 3, 4), that is needed for touch sensitivity. This last gene is also required for the function of other degenerins. Here we show that mec-6 encodes a single-pass membrane-spanning protein with limited similarity to paraoxonases, which are implicated in human coronary heart disease. This gene is expressed in muscle cells and in many neurons, including the six touch receptor neurons. MEC-6 increases amiloride-sensitive Na+ currents produced by MEC-4(d)/MEC-10(d) by ∼30-fold, and functions synergistically with MEC-2 (a stomatin-like protein that regulates MEC-4(d)/MEC-10(d) channel activity) to increase the currents by 200-fold. MEC-6 physically interacts with all three channel proteins. In vivo, MEC-6 colocalizes with MEC-4, and is required for punctate MEC-4 expression along touch-neuron processes. We propose that MEC-6 is a part of the degenerin channel complex that may mediate mechanotransduction in touch cells.

Original languageEnglish
Pages (from-to)669-673
Number of pages5
JournalNature
Volume420
Issue number6916
DOIs
StatePublished - 12 Dec 2002

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Degenerin Sodium Channels
Touch
Neurons
Proteins
Genes
Cellular Mechanotransduction
Epithelial Sodium Channels
Mutation
Amiloride
Caenorhabditis elegans
Muscle Cells
Coronary Disease
Mammals
Membrane Proteins
Phenotype

Cite this

Chelur, D. S., Ernstrom, G. G., Goodman, M. B., Yao, C. A., Chen, L., O'Hagan, R., & Chalfie, M. (2002). The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel. Nature, 420(6916), 669-673. https://doi.org/10.1038/nature01205
Chelur, Dattananda S. ; Ernstrom, Glen G. ; Goodman, Miriam B. ; Yao, C. Andrea ; Chen, Lei ; O'Hagan, Robert ; Chalfie, Martin. / The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel. In: Nature. 2002 ; Vol. 420, No. 6916. pp. 669-673.
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abstract = "Mechanosensory transduction in touch receptor neurons is believed to be mediated by DEG/ENaC (degenerin/epithelial Na+ channel) proteins in nematodes and mammals. In the nematode Caenorhabditis elegans, gain-of-function mutations in the degenerin genes mec-4 and mec-10 (denoted mec-4(d) and mec-10(d), respectively) cause degeneration of the touch cells. This phenotype is completely suppressed by mutation in a third gene, mec-6 (refs 3, 4), that is needed for touch sensitivity. This last gene is also required for the function of other degenerins. Here we show that mec-6 encodes a single-pass membrane-spanning protein with limited similarity to paraoxonases, which are implicated in human coronary heart disease. This gene is expressed in muscle cells and in many neurons, including the six touch receptor neurons. MEC-6 increases amiloride-sensitive Na+ currents produced by MEC-4(d)/MEC-10(d) by ∼30-fold, and functions synergistically with MEC-2 (a stomatin-like protein that regulates MEC-4(d)/MEC-10(d) channel activity) to increase the currents by 200-fold. MEC-6 physically interacts with all three channel proteins. In vivo, MEC-6 colocalizes with MEC-4, and is required for punctate MEC-4 expression along touch-neuron processes. We propose that MEC-6 is a part of the degenerin channel complex that may mediate mechanotransduction in touch cells.",
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Chelur, DS, Ernstrom, GG, Goodman, MB, Yao, CA, Chen, L, O'Hagan, R & Chalfie, M 2002, 'The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel', Nature, vol. 420, no. 6916, pp. 669-673. https://doi.org/10.1038/nature01205

The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel. / Chelur, Dattananda S.; Ernstrom, Glen G.; Goodman, Miriam B.; Yao, C. Andrea; Chen, Lei; O'Hagan, Robert; Chalfie, Martin.

In: Nature, Vol. 420, No. 6916, 12.12.2002, p. 669-673.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The mechanosensory protein MEC-6 is a subunit of the C. elegans touch-cell degenerin channel

AU - Chelur, Dattananda S.

AU - Ernstrom, Glen G.

AU - Goodman, Miriam B.

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AU - Chen, Lei

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AU - Chalfie, Martin

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