The Multipurpose 15-Protofilament Microtubules in C. elegans Have Specific Roles in Mechanosensation

Alexander Bounoutas, Robert O'Hagan, Martin Chalfie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Because microtubules perform many essential functions in neurons, delineating unique roles attributable to these organelles presents a formidable challenge. Microtubules endow neurons with shape and structure and are required for developmental processes including neurite outgrowth [1], intracellular transport [2], and synapse formation and plasticity [3, 4]; microtubules in sensory neurons may be required for the above processes in addition to a specific sensory function. In Caenorhabditis elegans, six touch receptor neurons (TRNs) sense gentle touch [5] and uniquely contain 15-protofilament microtubules [6]. Disruption of these microtubules by loss of either the MEC-7 β-tubulin [7] or MEC-12 α-tubulin [8] or by growth in 1 mM colchicine causes touch insensitivity [5, 6], altered distribution of the touch transduction channel, and a general reduction in protein levels. We show that the effect on touch sensitivity can be separated from the others; microtubule depolymerization in mature TRNs causes touch insensitivity but does not result in protein distribution and production defects. In addition, the mec-12(e1605) mutation selectively causes touch insensitivity without affecting microtubule formation and other cellular processes. Touching e1605 animals produces a reduced mechanoreceptor current that inactivates more rapidly than in wild-type, suggesting a specific role of the microtubules in mechanotransduction.

Original languageEnglish
Pages (from-to)1362-1367
Number of pages6
JournalCurrent Biology
Volume19
Issue number16
DOIs
StatePublished - 25 Aug 2009

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touch (sensation)
Touch
Microtubules
microtubules
Neurons
Tubulin
neurons
tubulin
Depolymerization
Colchicine
Plasticity
Animals
Proteins
mechanoreceptors
receptors
Mechanoreceptors
neurites
depolymerization
sensory neurons
Caenorhabditis elegans

Keywords

  • CELLBIO

Cite this

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abstract = "Because microtubules perform many essential functions in neurons, delineating unique roles attributable to these organelles presents a formidable challenge. Microtubules endow neurons with shape and structure and are required for developmental processes including neurite outgrowth [1], intracellular transport [2], and synapse formation and plasticity [3, 4]; microtubules in sensory neurons may be required for the above processes in addition to a specific sensory function. In Caenorhabditis elegans, six touch receptor neurons (TRNs) sense gentle touch [5] and uniquely contain 15-protofilament microtubules [6]. Disruption of these microtubules by loss of either the MEC-7 β-tubulin [7] or MEC-12 α-tubulin [8] or by growth in 1 mM colchicine causes touch insensitivity [5, 6], altered distribution of the touch transduction channel, and a general reduction in protein levels. We show that the effect on touch sensitivity can be separated from the others; microtubule depolymerization in mature TRNs causes touch insensitivity but does not result in protein distribution and production defects. In addition, the mec-12(e1605) mutation selectively causes touch insensitivity without affecting microtubule formation and other cellular processes. Touching e1605 animals produces a reduced mechanoreceptor current that inactivates more rapidly than in wild-type, suggesting a specific role of the microtubules in mechanotransduction.",
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The Multipurpose 15-Protofilament Microtubules in C. elegans Have Specific Roles in Mechanosensation. / Bounoutas, Alexander; O'Hagan, Robert; Chalfie, Martin.

In: Current Biology, Vol. 19, No. 16, 25.08.2009, p. 1362-1367.

Research output: Contribution to journalArticleResearchpeer-review

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