Uptake of bacteriophage λ DNA in Escherichia Coli by electroporation

An alternative to In Vitro packaging

John Gaynor, Martin G. Fox

Research output: Contribution to journalArticleResearchpeer-review

Abstract

By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage λ DNA, we obtained efficiencies of 1.1 × 106 (pfu/μg bacteriophage λ DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage λ DNA for the production of cDNA and genomic libraries.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalBiotechnology Techniques
Volume10
Issue number6
StatePublished - 1 Dec 1996

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Bacteriophages
Electroporation
Product Packaging
Escherichia coli
Packaging
DNA
Recombinant DNA
Genomic Library
Gene Library
Transfection
Shock
Hot Temperature
In Vitro Techniques

Cite this

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Uptake of bacteriophage λ DNA in Escherichia Coli by electroporation : An alternative to In Vitro packaging. / Gaynor, John; Fox, Martin G.

In: Biotechnology Techniques, Vol. 10, No. 6, 01.12.1996, p. 463-467.

Research output: Contribution to journalArticleResearchpeer-review

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