Abstract
By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage λ DNA, we obtained efficiencies of 1.1 × 106 (pfu/μg bacteriophage λ DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage λ DNA for the production of cDNA and genomic libraries.
| Original language | English |
|---|---|
| Pages (from-to) | 463-467 |
| Number of pages | 5 |
| Journal | Biotechnology Techniques |
| Volume | 10 |
| Issue number | 6 |
| State | Published - 1996 |