Visualizing cell extracellular matrix (ECM) deposited by cells cultured on aligned bacteriophage M13 thin films

Laying Wu, L. Andrew Lee, Zhongwei Niu, Soumitra Ghoshroy, Qian Wang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Topographical features ranging from micro- to nanometers can affect cell orientation and migratory pathways, which are important factors in tissue engineering and tumor migration. In our previous study, a convective assembly of bacteriophage M13 resulted in thin films which could be used to control the alignment of cells. However, several questions regarding its underlying reasons to dictate cell alignment remained unanswered. Here, we further study the nanometer topographical features generated by the bacteriophage M13 crystalline film, which results in the alignment of the cells and extracellular matrix (ECM) proteins. Sequential imaging analyses at micro- and nanoscale levels of aligned cells and fibrillar matrix proteins were documented using scanning electron microscopy and immunofluorescence microscopy. As a result, we observed baby hamster kidney cells with higher degree of alignment on the ordered M13 substrates than NIH-3T3 fibroblasts, a difference which could be attributed to the intrinsic nature of the cellś production of ECM proteins. The results from this study provide a crucial insight into the topographical features of a biological thin film, which can be utilized to control the orientation of cells and surrounding ECM proteins.

Original languageEnglish
Pages (from-to)9490-9496
Number of pages7
JournalLangmuir
Volume27
Issue number15
DOIs
StatePublished - 2 Aug 2011

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bacteriophages
Bacteriophages
cultured cells
Extracellular Matrix Proteins
Proteins
Thin films
matrices
thin films
cells
alignment
proteins
Fibroblasts
Tissue engineering
Tumors
Microscopic examination
Crystalline materials
Imaging techniques
Scanning electron microscopy
hamsters
tissue engineering

Cite this

Wu, Laying ; Lee, L. Andrew ; Niu, Zhongwei ; Ghoshroy, Soumitra ; Wang, Qian. / Visualizing cell extracellular matrix (ECM) deposited by cells cultured on aligned bacteriophage M13 thin films. In: Langmuir. 2011 ; Vol. 27, No. 15. pp. 9490-9496.
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Visualizing cell extracellular matrix (ECM) deposited by cells cultured on aligned bacteriophage M13 thin films. / Wu, Laying; Lee, L. Andrew; Niu, Zhongwei; Ghoshroy, Soumitra; Wang, Qian.

In: Langmuir, Vol. 27, No. 15, 02.08.2011, p. 9490-9496.

Research output: Contribution to journalArticle

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