Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice

Shengmin Sang, Jihyeung Ju, Joshua D. Lambert, Yong Lin, Jungil Hong, Mousumi Bose, Steven Wang, Naisheng Bai, Kan He, Bandaru S. Reddy, Chi Tang Ho, Frederick Li, Chung S. Yang

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.

Original languageEnglish
Pages (from-to)9792-9797
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number26
DOIs
Publication statusPublished - 27 Dec 2006

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Keywords

  • Apc mice
  • Colon cancer
  • Nonpolar lipids
  • Phytochemicals
  • Wheat bran oil

Cite this

TY - JOUR

T1 - Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice

AU - Sang, Shengmin

AU - Ju, Jihyeung

AU - Lambert, Joshua D.

AU - Lin, Yong

AU - Hong, Jungil

AU - Bose, Mousumi

AU - Wang, Steven

AU - Bai, Naisheng

AU - He, Kan

AU - Reddy, Bandaru S.

AU - Ho, Chi Tang

AU - Li, Frederick

AU - Yang, Chung S.

PY - 2006/12/27

Y1 - 2006/12/27

N2 - This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.

AB - This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.

KW - Apc mice

KW - Colon cancer

KW - Nonpolar lipids

KW - Phytochemicals

KW - Wheat bran oil

UR - http://www.scopus.com/inward/record.url?scp=33846486976&partnerID=8YFLogxK

U2 - 10.1021/jf0620665

DO - 10.1021/jf0620665

M3 - Article

C2 - 17177503

AN - SCOPUS:33846486976

VL - 54

SP - 9792

EP - 9797

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 26

ER -