Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice

Shengmin Sang, Jihyeung Ju, Joshua D. Lambert, Yong Lin, Jungil Hong, Mousumi Bose, Steven Wang, Naisheng Bai, Kan He, Bandaru S. Reddy, Chi Tang Ho, Frederick Li, Chung S. Yang

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.

Original languageEnglish
Pages (from-to)9792-9797
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number26
DOIs
StatePublished - 27 Dec 2006

Fingerprint

Dietary Fiber
Cell growth
wheat bran
colorectal neoplasms
Colonic Neoplasms
carcinogenesis
cell growth
Oils
Carcinogenesis
oils
mice
Growth
Phytochemicals
animal models
phytopharmaceuticals
Phytosterols
Cells
phytosterols
cell lines
Intestinal Neoplasms

Keywords

  • Apc mice
  • Colon cancer
  • Nonpolar lipids
  • Phytochemicals
  • Wheat bran oil

Cite this

Sang, Shengmin ; Ju, Jihyeung ; Lambert, Joshua D. ; Lin, Yong ; Hong, Jungil ; Bose, Mousumi ; Wang, Steven ; Bai, Naisheng ; He, Kan ; Reddy, Bandaru S. ; Ho, Chi Tang ; Li, Frederick ; Yang, Chung S. / Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice. In: Journal of Agricultural and Food Chemistry. 2006 ; Vol. 54, No. 26. pp. 9792-9797.
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title = "Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice",
abstract = "This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2{\%} WB oil significantly inhibited the overall tumorigenesis by 35.7{\%} (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.",
keywords = "Apc mice, Colon cancer, Nonpolar lipids, Phytochemicals, Wheat bran oil",
author = "Shengmin Sang and Jihyeung Ju and Lambert, {Joshua D.} and Yong Lin and Jungil Hong and Mousumi Bose and Steven Wang and Naisheng Bai and Kan He and Reddy, {Bandaru S.} and Ho, {Chi Tang} and Frederick Li and Yang, {Chung S.}",
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Sang, S, Ju, J, Lambert, JD, Lin, Y, Hong, J, Bose, M, Wang, S, Bai, N, He, K, Reddy, BS, Ho, CT, Li, F & Yang, CS 2006, 'Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice', Journal of Agricultural and Food Chemistry, vol. 54, no. 26, pp. 9792-9797. https://doi.org/10.1021/jf0620665

Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice. / Sang, Shengmin; Ju, Jihyeung; Lambert, Joshua D.; Lin, Yong; Hong, Jungil; Bose, Mousumi; Wang, Steven; Bai, Naisheng; He, Kan; Reddy, Bandaru S.; Ho, Chi Tang; Li, Frederick; Yang, Chung S.

In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 26, 27.12.2006, p. 9792-9797.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Wheat bran oil and its fractions inhibit human colon cancer cell growth and intestinal tumorigenesis in Apcmin/+ mice

AU - Sang, Shengmin

AU - Ju, Jihyeung

AU - Lambert, Joshua D.

AU - Lin, Yong

AU - Hong, Jungil

AU - Bose, Mousumi

AU - Wang, Steven

AU - Bai, Naisheng

AU - He, Kan

AU - Reddy, Bandaru S.

AU - Ho, Chi Tang

AU - Li, Frederick

AU - Yang, Chung S.

PY - 2006/12/27

Y1 - 2006/12/27

N2 - This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.

AB - This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. We studied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model, a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines (HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oil fraction of WB was more active than the water fraction against the growth of human colon cancer cell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001) in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicals and the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk-(en) ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography. Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growth inhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in the Apcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancer preventive activity of WB oil. The active ingredients, however, remain to be identified.

KW - Apc mice

KW - Colon cancer

KW - Nonpolar lipids

KW - Phytochemicals

KW - Wheat bran oil

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U2 - 10.1021/jf0620665

DO - 10.1021/jf0620665

M3 - Article

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SP - 9792

EP - 9797

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SN - 0021-8561

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ER -